NR ARBA
AU Reuter,A.; Binkle,U.; Stuermer,C.A.O.; Plattner,H.
TI PrPc and reggies/flotillins are contained in and released via lipid-rich vesicles in Jurkat T cells
QU Cellular and Molecular Life Science 2004 Aug; 61(16): 2092-9
PT journal article
AB A new model of caveolin association with lipid body cores has recently been proposed which may be relevant to a number of cellular processes, e.g. lipid body generation. Here we show that PrPc and reggie-1 and reggie-2 also occur in the cores of Nile Red/Bodipy-stained (neutral lipid-containing) vesicular structures and, in immunoblots, in the lipid-enriched fraction after density gradient centrifugation. These lipid-rich vesicles increase in number following cell feeding with oleic acid, differ from early endosome antigen 1- and Lamp-2-positive endosomes/lysosomes, exhibit an opaque content and lack surrounding actin staining. Our results suggest that the content of these vesicles, together with reggie-1 and -2 and PrPc, is expelled.
MH Actins/metabolism; Cytoplasmic Vesicles/*metabolism/ultrastructure; Fluorescent Dyes; Human; Immunohistochemistry; Jurkat Cells; Membrane Proteins/*metabolism; Microscopy, Electron; Oxazines; PrPc Proteins/*metabolism; Staining and Labeling; Support, Non-U.S. Gov't
AD Department of Biology, University of Konstanz, 78457, Konstanz, Germany. alexander.reuter@uni-konstanz.de
SP englisch
PO Schweiz