NR ARCZ
AU Vorberg,I.; Gilch,S.; Kehler,C.; Priola,S.A.; Schätzl,H.M.
TI Cell culture models for prion diseases
QU TSE-Forum, 4. Kongress - Nationale TSE-Forschungsplattform, Düsseldorf 28.10.-29.10.2004, Vortrag V-16
PT Konferenz-Vortrag
AB The development of cell culture models for prion diseases has greatly enhanced our understanding of the molecular mechanism of PrPsc formation and has helped to identify potential anti-prion compounds. While few cell cultures have been identified that can be persistently infected with prions, the cellular requirements for PrPsc formation and infection remain elusive. Successful infections of a variety of cell lines demonstrate that the susceptibility of a given cell line can not be predicted based on its tissue origin. Furthermore, there is increasing evidence that over-expression of cellular prion protein does not necessarily increase PrPsc formation and prion susceptibility. By using different cell culture lines we show here that transient PrPsc formation can be initiated in a variety of PrPc expressing cells, while the establishment of a productive prion infection appears to be restricted to few cell cultures that potentially express additional cell- and/or scrapie strain-specific factors for the scrapie agent to adapt to and persistently infect a cell.
AD Ina Vorberg, Sabine Gilch, Claudia Kehler, Hermann Schätzl, Institute of Virology, Technical University of Munich, Biedersteinerstr. 29, 80802 Munich; Sue Priola, Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton MT 59840, USA
SP englisch
PO Deutschland
OR Tagungsband