NR ARDP
AU Xiang,W.; Windl,O.; Wünsch,G.; Dugas,M.; Kohlmann,A.; Westner,I.M.; Kretzschmar,H.A.
TI Expression profiling in scrapie-infected mouse brains by using high-density oligonucleotide probe microarrays
QU TSE-Forum, 4. Kongress - Nationale TSE-Forschungsplattform, Düsseldorf 28.10.-29.10.2004, Poster PATH-03
PT Konferenz-Poster
AB The aim of this study was to identify the differentially regulated genes that correlate with the development of prion disease for a better understanding of the pathological mechanisms. We applied the high-density oligonucleotide probe microarrays of Affymetrix (Mouse Expression Array 430A) containing more than 22,000 transcripts and compared the global gene expression profiles from brains of mice intracerebrally inoculated with scrapie strains ME7 and RML with those from brains of uninfected and mock-infected mice. Microarray data were analyzed by Significance Analysis of Microarrays (SAM), revealing 121 genes whose expression increased at least twofold in both ME7- and RML-infected mouse brains with an estimated false discovery rate of <= 5%. These genes encode proteins involved in proteolysis, protease inhibition, cell growth/maintenance, immune response, signal transduction, cell adhesion, and molecular metabolism. The time course of the expression showed generally up-regulation in these genes from 120 days post inoculation (dpi) in ME7-inoculated mouse brains and 90 dpi in RML-inoculated mouse brains. The onset of the elevated expression correlated temporally with the onset of the PrPsc accumulation. As the most of these genes are described in the context of scrapie for the first time, they may represent novel potential diagnostic and therapeutic targets for prion disease.
AD Wei Xiang, Otto Windl, Gerda Wünsch, Ingo M. Westner and Hans A. Kretzschmar, Institute of Neuropathology, Ludwig-Maximilians-University Munich, Munich, Germany; Martin Dugas, Department of Medical Informatics, Biometrics, and Epidemiology, Ludwig-Maximilians-University, Munich, Germany; Alexander Kohlmann, Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany
SP englisch
PO Deutschland
OR Tagungsband