NR ARHN
AU Haynes,C.M.; Titus,E.A.; Cooper,A.A.
TI Degradation of misfolded proteins prevents ER-derived oxidative stress and cell death
QU Molecular Cell 2004 Sep 10; 15(5): 767-76
PT journal article
AB A variety of debilitating diseases including diabetes, Alzheimer's, Huntington's, Parkinson's, and prion-based diseases are linked to stress within the endoplasmic reticulum (ER). Using S. cerevisiae, we sought to determine the relationship between protein misfolding, ER stress, and cell death. In the absence of ERV29, a stress-induced gene required for ER associated degradation (ERAD), misfolded proteins accumulate in the ER leading to persistent ER stress and subsequent cell death. Cells alleviate ER stress through the unfolded protein response (UPR); however, if stress is sustained the UPR contributes to cell death by causing the accumulation of reactive oxygen species (ROS). ROS are generated from two sources: the UPR-regulated oxidative folding machinery in the ER and mitochondria. Our results demonstrate a direct mechanism(s) by which misfolded proteins lead to cellular damage and death.
MH Cell Death/physiology; Cells, Cultured; Endoplasmic Reticulum/*metabolism; Energy Metabolism/physiology; Membrane Proteins/genetics/metabolism; Mitochondria/metabolism; Oxidative Stress/*physiology; *Protein Folding; Reactive Oxygen Species/*metabolism; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Saccharomyces cerevisiae/*metabolism; Saccharomyces cerevisiae Proteins/genetics/*metabolism
AD Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA
SP englisch
PO USA