NR ARHS
AU Jin,J.K.; Jeong,B.H.; Na,Y.J.; Kim,Y.S.; Carp,R.I.; Wie,M.B.; Moon,C.; Shin,T.
TI Increased expression of the embryonic intermediate filament, nestin, in the brains of scrapie-infected mice
QU Neuroscience Letters 2004 Sep 2; 367(2): 254-8
PT journal article
AB This study investigated the immunohistochemical localization of nestin and its expression level in the brains of mice infected with scrapie, a disease which is characterized by spongiform neurodegeneration, neuronal vacuolation, and astrogliosis. Western blot analysis showed that the protein level of nestin was significantly increased in scrapie-infected brains compared with those of control brains. Nestin immunoreactivity in vascular endothelial cells was more intense in the brains of scrapie-infected mice compared with those of controls. There was marked nestin immunostaining in reactive astrocytes, but not in the neurons of the scrapie-infected brains. Considering all the findings, this study suggests that the nestin-positive cells, including glial cells and vascular endothelial cells, may have the potential to differentiate into mature glial cells and other neuronal elements, which would replenish lost neurons.
MH Animals; Blotting, Western/methods; Brain/*metabolism/pathology; Comparative Study; Gene Expression Regulation, Developmental/*physiology; Glial Fibrillary Acidic Protein/metabolism; Immunohistochemistry/methods; Intermediate Filament Proteins/*metabolism; Intermediate Filaments/*metabolism; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins/*metabolism; RNA, Messenger/biosynthesis; Research Support, Non-U.S. Gov't; Reverse Transcriptase Polymerase Chain Reaction/methods; Scrapie/*metabolism
AD Ilsong Institute of Life Science, Hallym University, Ilsong Building, Dongan-gu, Anyang 431-060, South Korea.
SP englisch
PO Irland