NR ARHU
AU Kawamoto,Y.; Akiguchi,I.; Jarius,C.; Budka,H.
TI Enhanced expression of 14-3-3 proteins in reactive astrocytes in Creutzfeldt-Jakob disease brains
QU Acta Neuropathologica 2004 Oct; 108(4): 302-8
PT journal article
AB 14-3-3 proteins have been reported to be detected specifically in the cerebrospinal fluid (CSF) from patients with Creutzfeldt-Jakob disease (CJD). To elucidate the role of 14-3-3 proteins in patients with CJD, we performed immunohistochemical studies on 14-3-3 proteins in autopsied brains from five patients with sporadic CJD (sCJD), three patients with Alzheimer's disease (AD), and seven normal control subjects. Formalin-fixed, paraffin-embedded sections from all cases were immunostained with several types of specific anti-14-3-3 antibodies. In the normal control brains, 14-3-3 immunoreactivity was localized mainly in the neuronal somata and processes; in contrast, glial cells showed no or faint immunoreactivity. In the brains from the patients with AD, 14-3-3 immunoreactivity was observed in the surviving neurons as well as some neurofibrillary tangles. In the brains from the patients with sCJD, 14-3-3 immunoreactivity was well preserved in the remaining neurons. Furthermore, the glial cells, especially the reactive astrocytes, were intensely immunostained in the brains affected by sCJD. Our findings suggest that 14-3-3 proteins may be up-regulated in the glial cells, particularly in reactive astrocytes, and that the enhanced expression of 14-3-3 proteins in these glial elements may be associated with the pathogenesis of sCJD.
MH 14-3-3 Proteins/*biosynthesis; Aged; Alzheimer Disease/metabolism/pathology; Astrocytes/*metabolism/pathology; Brain/metabolism/*pathology; Comparative Study; Creutzfeldt-Jakob Syndrome/metabolism/*pathology; Female; Humans; Immunohistochemistry; Male; Middle Aged; Neurofibrillary Tangles/metabolism/pathology; Neurons/metabolism/pathology; Research Support, Non-U.S. Gov't
AD Department of Neurology, Faculty of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Sakyoku, 606-8507 Kyoto, Japan. kawamoto@kuhp.kyoto-u.ac.jp
SP englisch
PO Deutschland