NR ARMH
AU Gofflot,S.; El Moualij,B.; Zorzi,D.; Melen,L.; Roels,S.L.M.F.; Quatpers,D.; Grassi,J.; Vanopdenbosch,E.; Heinen,E.; Zorzi,W.
TI Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein
QU Journal of Immunoassay and Immunochemistry 2004; 25(3): 241-58
PT journal article
AB Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR (iqPCR), exploiting real-time PCR technology, in order to improve this immuno-detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting the cellular and the resistant form of prion protein in bovine brain extract. The iqPCR technique proved to be more sensitive than ELISA, so it could be a technique of choice for the diagnosis of infected animals both at an ante mortem and post-mortem stage.
MH Animals; Antibodies, Monoclonal/*chemistry/immunology; *Brain Chemistry; Cattle; Enzyme-Linked Immunosorbent Assay/methods; Hamsters; Humans; Polymerase Chain Reaction/*methods; Prions/*analysis/immunology; Research Support, Non-U.S. Gov't; Sensitivity and Specificity
AD Stéphanie Gofflot, Benaïssa El Moualij, Danièle Zorzi, Laurence Melen, Ernst Heinen, Willy Zorzi, Service d'Histologie Humaine-Centre de Recherche sur les Protéines Prions (CRPP), Université de Liège-CHU, Tour de Pharmacie, Avenue de l'Hôpital 1, B-4000, Liège, Belgium; Stefan Roels, Emmanuel Vanopdenbosch, VAR-CERVA-CODA, Veterinary and Agrochemical Research Center, Department of Biocontrol-Section of Pathology, National Reference Laboratory for Veterinary TSE (Belgium & Luxemburg), Brussels, Belgium; Dominique Quatpers, CRPP-Laboratoire ULg BSE, Tour de Pharmacie, Liège, Belgium; Jacques Grassi, CEA, Service de Pharmacologie et d'Immunologie, CEA Saclay, Gif sur Yvette, France
SP englisch
PO USA