NR ARNT
AU Bate,C.; Williams,A.
TI Detoxified lipopolysaccharide reduces microglial cell killing of prion-infected neurons
QU Neuroreport 2004 Dec 22; 15(18): 2765-8
PT journal article
AB In vitro studies show that microglial cells kill neurons treated with the synthetic miniprion (sPrP106) or with amyloid-beta1-42 (a neurotoxic peptide found in Alzheimer's disease) by a process requiring the CD14 protein. The killing of treated primary cortical neurons by microglial cells was reduced by the addition of detoxified lipopolysaccharide (LPS), a deacylated form of LPS. Detoxified LPS also increased the survival of prion-infected neuroblastoma cells incubated with microglial cells. The presence of detoxified LPS reduced cytokine production in these co-cultures, and from isolated microglial cells incubated with native LPS, or fibrils of sPrP106 or amyloid-beta1-42. These results suggest that some compounds that bind to CD14 might reduce microglial cell activation and increase neuronal survival in prion and Alzheimer's diseases.
MH Amyloid beta-Protein/pharmacology; Analysis of Variance; Animals; Animals, Newborn; Cell Survival/drug effects; Cells, Cultured; Coculture Techniques/methods; Comparative Study; Cytokines/metabolism; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay/methods; Infection/therapy; Lipopolysaccharides/*pharmacology; Metabolic Detoxication, Drug; Mice; Microglia/*drug effects/physiology; Neuroblastoma; Neurons/cytology/*drug effects; Peptide Fragments/pharmacology; Peptides/pharmacology; Prions/*pharmacology; Research Support, Non-U.S. Gov't; Tetrazolium Salts/diagnostic use
AD Department of Veterinary Pathology, Glasgow University Veterinary School, Bearsden Road, Glasgow G61 1QH, UK. c.bate@vet.gla.ac.uk
SP englisch
PO England