NR ARTT
AU Toni,M.; Massimino,M.L.; Griffoni,C.; Salvato,B.; Tomasi,V.; Spisni,E.
TI Extracellular copper ions regulate cellular prion protein (PrPc) expression and metabolism in neuronal cells
QU FEBS Letters 2005 Jan 31; 579(3): 741-4
PT journal article
AB The physiological functions of cellular prion protein (PrPc) remain unclear. It has been demonstrated that PrPc is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPc expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPc mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPc into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPc in neurones and its conversion in the pathological isoform PrPsc.
MH Animals; Blotting, Northern; Blotting, Western; Cell Line; Copper/*physiology; Mice; Neurons/*metabolism; PrPc Proteins/*genetics/*metabolism; RNA, Messenger/genetics; Research Support, Non-U.S. Gov't
AD Department of Experimental Biology, University of Bologna, Via Selmi 3, 40126 Bologna, Italy.
SP englisch
PO Niederlande