NR ASBY
AU Jeziore,Y.; Thompson,E.J.
TI Limitations of dot-blot immunoassay in the diagnosis of Creutzfeldt-Jakob disease
QU Neuroscience Research Communications 1995 May-Jun; 16(3): 163-71
PT Article
AB Dot-blot immunoassay has been used in prion disease research and we wanted to see if this technique could be used to screen cerebrospinal fluid for Creutzfeldt-Jakob disease. We found that the high background staining arising from non- specific binding of antibodies to the sample spots, even after proteinase K digestion prevented any observable distinction between positive Creutzfeldt-Jakob disease tissue and controls. Densitometric scanning distinguished some positive cases more reliably than the human eye but the results were not always consistent because of the high background staining. We therefore conclude that dot-blot immunoassay is not a reliable technique for the diagnosis of Creutzfeldt-Jakob disease.
IN Die Autoren konnten aufgrund starker Hintergrundmarkierung keinen zuverlässigen Dot-blot-Immunoassay für eine Diagnose der Creutzfeldt-Jakob-Krankheit unter Verwendung von Rückenmarksflüssigkeit entwickeln.
ZR 27
AD Y. Jeziore, Inst Neurol, Dept Neurochem, Queen Sq, London WC1N 3BG, England
SP englisch