NR ASLK
AU Seabury,C.M.; Halbert,N.D.; Gogan,P.J.; Templeton,J.W.; Derr,J.N.
TI Bison PRNP genotyping and potential association with Brucella spp. seroprevalence.
QU Animal Genetics 2005 Apr; 36(2): 104-10
PT journal article
AB The implication that host cellular prion protein (PrPc) may function as a cell surface receptor and/or portal protein for Brucella abortus in mice prompted an evaluation of nucleotide and amino acid variation within exon 3 of the prion protein gene (PRNP) for six US bison populations. A non-synonymous single nucleotide polymorphism (T50C), resulting in the predicted amino acid replacement M17T (Met --> Thr), was identified in each population. To date, no variation (T50; Met) has been detected at the corresponding exon 3 nucleotide and/or amino acid position for domestic cattle. Notably, 80% (20 of 25) of the Yellowstone National Park bison possessing the C/C genotype were Brucella spp. seropositive, representing a significant (P = 0.021) association between seropositivity and the C/C genotypic class. Moreover, significant differences in the distribution of PRNP exon 3 alleles and genotypes were detected between Yellowstone National Park bison and three bison populations that were either founded from seronegative stock or previously subjected to test-and-slaughter management to eradicate brucellosis. Unlike domestic cattle, no indel polymorphisms were detected within the corresponding regions of the putative bison PRNP promoter, intron 1, octapeptide repeat region or 3'-untranslated region for any population examined. This study provides the first evidence of a potential association between nucleotide variation within PRNP exon 3 and the presence of Brucella spp. antibodies in bison, implicating PrPc in the natural resistance of bison to brucellosis infection.
MH Age Factors; Amyloid/*genetics; Animals; Antibodies, Bacterial/*blood; Bison/*genetics; Brucella/*immunology; Comparative Study; Female; Gene Frequency; Genotype; Geography; Male; Protein Precursors/*genetics; Sequence Analysis, DNA; Seroepidemiologic Studies; Sex Factors; United States
AD Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843-4467, USA
SP englisch
PO England