NR ASOF
AU Brown,P.; Rau,E.H.; Lemieux,P.; Johnson,B.K.; Bacote,A.E.; Gajdusek,D.C.
TI Infectivity studies of both ash and air emissions from simulated incineration of scrapie-contaminated tissues
QU Environmental Science and Technology 2004 Nov 15; 38(22): 6155-60
PT journal article
AB We investigated the effectiveness of 15 min exposures to 600 and 1000 degrees C in continuous flow normal and starved-air incineration-like conditions to inactivate samples of pooled brain macerates from hamsters infected with the 263K strain of hamster-adapted scrapie with an infectivity titer in excess of 10(9) mean lethal doses (LD50) per g. Bioassays of the ash, outflow tubing residues, and vented emissions from heating 1 g of tissue samples yielded a total of two transmissions among 21 inoculated animals from the ash of a single specimen burned in normal air at 600 degrees C. No other ash, residue, or emission from samples heated at either 600 or 1000 degrees C, under either normal or starved-air conditions, transmitted disease. We conclude that at temperatures approaching 1000 degrees C under the air conditions and combustion times used in these experiments, contaminated tissues can be completely inactivated, with no release of infectivity into the environment from emissions. The extent to which this result can be realized in actual incinerators and other combustion devices will depend on equipment design and operating conditions during the heating process.
IN Zwei von 21 Hamstern erkrankten nach der Inokulation von Asche, die nach 15 Minuten bei 600° von je 1 g Hamsterhirn übrig geblieben war, welches mehr als 1 Milliarde LD50(Hamster) des an Hamster adaptierten Scrapiestammes 263K enthielt. Weil sie nach 15 Minuten bei 1000° keine Infektiosität mehr fanden, meinen die Autoren, dass TSE-kontaminiertes Material bei 1000° vollständig inaktiviert werden könne.
MH Air Pollutants, Environmental; Animals; Brain/metabolism; Hamsters; *Heat; Incineration/*methods; PrPsc Proteins/*pathogenicity; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Scrapie/*transmission; Time Factors
AD Laboratory of CNS Studies, National Institute of Neurological Disorders and Stroke, and Div. of Environmental Protection, Office of Research Facilities Development and Operations, NIH, US Dept. of HHS, Bethesda, MD 20892, USA. Paul Brown (paulwbrown@comcast.net)
SP englisch
PO USA
ZF Zusammenfassung des Abstracts von Roland Heynkes