NR ASPQ
AU Kariv-Inbal,Z.; Halimi,M.; Dayan,Y.; Engelstein,R.; Gabizon,R.
TI Characterization of light chain immunoglobulin in urine from animals and humans infected with prion diseases
QU Journal of Neuroimmunology 2005 May; 162(1-2): 12-8
PT journal article
AB The necessity of a non-invasive in-vivo test for prion diseases has become more apparent since the transmission of vCJD from the blood of a healthy individual incubating the disease. Here we show that prion urine comprises an array of protease resistant peptides, among them light chain immunoglobulin (LC). This was observed by sequencing gel bands comprising hamster urine samples, as well as by immunoblotting of similar samples with anti mouse IgG reagents for hamster samples, or with anti human IgG reagents for human samples. Our result suggests that urine samples from CJD patients can be identified by the presence of protease resistant proteins such as LC.
IN Im Urin von Scrapie-infizierten Hamstern und Patienten mit der Creutzfeldt-Jakob-Krankheit fanden die Autoren Protease-resistente Proteinen, unter denen auch die leichten Ketten von Immunglobulinen waren.
MH Amino Acid Sequence; Animals; Bacterial Outer Membrane Proteins/metabolism; Bence Jones Protein/metabolism; Blotting, Western/methods; Comparative Study; Creutzfeldt-Jakob Syndrome/*immunology/*urine; Dialysis/methods; Hamsters; Humans; Immunoglobulins, Light-Chain/drug effects/*urine; Peptide Hydrolases/pharmacology; Spectrum Analysis, Mass/methods; Ultracentrifugation/methods
AD Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah University Hospital, Jerusalem 91120, Israel.
SP englisch
PO Niederlande