NR ASRG
AU Sharpe,A.; McElroy,M.C.; Langeveld,J.P.M.; Bassett,H.F.; O'Donoghue,A.M.; Sweeney,T.
TI Immunohistochemical studies of scrapie archival material from Irish ARQ/ARQ sheep for evidence of bovine spongiform encephalopathy-derived disease
QU Research in Veterinary Science 2005 Aug; 79(1): 29-35
PT journal article
AB Since scrapie and bovine spongiform encephalopathy (BSE) in sheep are clinicopathologically indistinguishable, BSE in sheep may have been misdiagnosed as scrapie. Disease-specific prion protein (PrP(d)) patterns in archival tissues of 38 Irish ARQ/ARQ sheep diagnosed as scrapie-affected were compared to those in four Dutch BSE-challenged sheep. When medulla oblongata was immunolabelled with an antibody directed against amino acids 93-99 of ovine prion protein (ovPrP), intraneuronal PrP(d) was apparent in all 38 Irish sheep but was absent in BSE-challenged sheep. When lymphoid follicles were immunolabelled with antibodies directed against amino acids 93-106 of ovPrP, granule clusters of PrP(d) were seen in 34 of the 38 Irish sheep. Follicles of the remaining four archive sheep contained either no PrP(d) or single PrP(d) granules, similar to follicles from BSE-challenged sheep. Based on the medulla results, none of the archival cases had BSE-derived disease. The identification of some scrapie sheep with little or no intrafollicular PrP(d) suggests that this technique may be limited in discriminating between the two diseases.
IN Die Autoren untersuchten archiviertes Material von irischen Scrapie-Fällen und fanden keine Hinweise auf BSE-Infektionen.
MH Animals; Cattle; Comparative Study; Diagnosis, Differential; Encephalopathy, Bovine Spongiform/*diagnosis/transmission; Immunohistochemistry/*veterinary; Ireland; Lymphoid Tissue/chemistry; Medulla Oblongata/chemistry; PrPsc Proteins/*analysis; Research Support, Non-U.S. Gov't; Sheep; Sheep Diseases/*transmission; Species Specificity
AD Ann Sharp (ann.sharpe@agriculture.gov.ie), Máire C. McElroy, Pathology Division, Central Veterinary Research Laboratory, Abbotstown, Castleknock, Dublin 15, Ireland
SP englisch
PO England