NR ASTY
AU Oboznaya,M.B.; Vol'pina,O.M.; Zhmak,M.N.; Titova,M.A.; Volkova,T.D.; Egorov,A.A.; Rybakov,S.S.; Ivanov,V.T.
TI [Induction of immune response by synthetic fragments of the bovine prion protein and their analogues in mice of various lines]
OT Induktsiia immunogo otveta sinteticheskimi fragmentami prionnogo belka i ikh analogami u myshei raznykh linii
QU Bioorganicheskaia Khimiia 2004 Jul-Aug; 30(4): 356-63
PT journal article
AB The antibodies to the bovine prion protein were produced by immunizing mice of three lines with five synthetic fragments of the protein and their six analogues. The analogues contained the amino acid substitutions that, according to theoretical calculation, should lead to an increase in the immunogenic activity of peptides. All the peptides, except for one, induced the formation of antibodies. All the sera containing the antipeptide antibodies were tested by an immunohistochemical method. The sera that were effectively bound to the brain preparations from the bovine with spongiform encephalopathy were identified; it was shown that they do not interact with the preparations of normal brain. Therefore, it was shown that the immunization of mice with the synthetic fragments of a prion protein helps obtain specific antibodies suitable for the study and diagnostics of prion diseases.
MH Amino Acid Sequence; Amino Acid Substitution; Animals; Antibodies, Monoclonal/*immunology; Brain/*immunology; Cattle; Encephalopathy, Bovine Spongiform/*immunology; English Abstract; Immune Sera/immunology; Immunization; Immunoenzyme Techniques; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred CBA; Molecular Sequence Data; Peptide Fragments/chemical synthesis/*immunology; Prions/*immunology; Species Specificity
SP russisch
PO Russland