NR ASWM
AU Coughlan,C.M.; Brodsky,J.L.
TI Use of yeast as a model system to investigate protein conformational diseases
QU Molecular Biotechnology 2005 Jun; 30(2): 171-80
PT journal article; review; review, tutorial
AB Protein conformational diseases arise when a cellular protein adopts an aberrant shape that either directly or indirectly alters the physiology of its host cell. Notable conformational diseases include cystic fibrosis, Huntington's disease, the prion-related diseases, Alzheimer's disease, and antitrypsin deficiency. In principle, the severity and progression of conformational diseases can be altered by cellular factors that recognize and attempt to ameliorate the harmful effects of the disease-causing, misshapen protein. To better define the mechanistic underpinnings of cellular factors that mediate quality control, and to understand why a single misfolded protein can impact cell viability, specific proteins that cause each of the diseases listed above have been expressed in a model eukaryote, the yeast Saccharomyces cerevisiae. In this review, we describe what has been learned from these studies, and speculate on future uses of yeast expression systems.
ZR 92
MH Animals; Cystic Fibrosis/genetics/*metabolism; Humans; Neurodegenerative Diseases/genetics/*metabolism; *Protein Conformation; Protein Folding; Saccharomyces cerevisiae/genetics/*metabolism
AD Department of Biological Sciences, University of Denver, Denver, CO 80208, USA
SP englisch
PO USA