NR ASWW
AU Grobben,A.H.; Steele,P.J.; Somerville,R.A.; Taylor,D.M.; Schreuder,B.E.C.
TI Inactivation of the BSE agent by the heat and pressure process for manufacturing gelatine
QU The Veterinary Record 2005 Sep 3; 157(10): 277-81
PT journal article
AB Dietary exposure to the bovine spongiform encephalopathy (BSE) agent is the probable cause of variant Creutzfeldt-Jakob disease in people. The industrial manufacturing process for the production of gelatine and colloidal protein by the heat and pressure process was downscaled accurately and its capacity to remove or inactivate bse infectivity was investigated. Gelatine was made from bones experimentally contaminated with mouse brain infected with the 301V strain of mouse-passaged bse agent in which the infective titre was 10(8.7) ID50/g. No infectivity was detected in the extracted protein (> or =10(0.45) ID50/g), and the calculated clearance factor was 10(6.5) ID50 or more.
IN Die Autoren liefern endlich die lange erwartete Auswertung der im Auftrag der europäischen Gelatine-Industrie durchgeführten Studien zum Ausmaß der Entfernung bzw. Inaktivierung von TSE-Erregern durch die Gelatine-Herstellungsprozesse. Demnach nimmt eventuell im Rohmaterial vorhandene Infektiosität während der Gelatine-Herstellung insgesamt mindestens um sechseinhalb Größenordnungen ab.
MH Animals; Bone and Bones/*chemistry; Cattle; Creutzfeldt-Jakob Syndrome/prevention & control; Encephalopathy, Bovine Spongiform/*transmission; Gelatin/*chemistry; Heat; Humans; Mice; Pressure; Prions/*pathogenicity; Research Support, Non-U.S. Gov't
AD Delft Gelatin, 4731 wh Oudenbosch, The Netherlands.
SP englisch
PO England