NR ASXS
AU Rosicarelli,B.; Serafini,B.; Sbriccoli,M.; Lu,M.; Cardone,F.; Pocchiari,M.; Aloisi,F.
TI Migration of dendritic cells into the brain in a mouse model of prion disease
QU Journal of Neuroimmunology 2005 Aug; 165(1-2): 114-20
PT journal article
AB The immune system plays a key role in the dissemination of prion infections from the periphery to the central nervous system (CNS). While follicular dendritic cells are critical for prion replication in lymphoid tissue and subsequent neuroinvasion, myeloid dendritic cells (DCs) have been implicated in both the clearance and propagation of pathological prion protein. Since nothing is known on the ability of DCs to migrate to the CNS during prion diseases, we investigated the immunohistochemical localization of CD205(+) DCs in the brain of C57BL/6 mice intraperitoneally infected with the mouse-adapted KFu strain of Gerstmann-Sträussler-Scheinker syndrome, a human genetic prion disorder. In normal brain, CD205(+) cells were present in the meninges and choroid plexus, whereas in the majority of mice sacrificed between 120 and 300 days post infection, CD205(+) DCs were also detected in the cerebral cortex, subcortical white matter, thalamus and medulla oblongata. These findings demonstrate that DCs can enter the CNS of prion-infected mice, suggesting a possible role for these cells in the pathogenesis of prion disorders.
IN Die Autoren fanden myeloide dendritische Zellen bei normalen C57BL/6-Mäusen in den harten Hirnhäuten (Meninges encephali) und dem Adergeflecht (Choroid plexus), 120-300 Tage nach einer intraperitonealen Inokulation mit dem mausadaptierten Gerstmann-Sträußler-Scheinker-Sydrom-Erregerstamm KFu jedoch in den meisten Mäusen auch in Hirnrinde (cerebral cortex), Marklager (subcortical white matter), Thalamus und Nachhirn (Medulla oblongata). Da myeloide dendritische Zellen PrPsc aufnehmen können, könnte dies ein denkbarer Weg der Infektiosität ins Gehirn sein.
MH Animals; Antigens, CD/biosynthesis; Brain/*immunology/metabolism/*pathology; Cell Movement/*immunology; Dendritic Cells/immunology/metabolism/*pathology; Disease Models, Animal; Female; Gerstmann-Sträussler-Scheinker Disease/*immunology/*pathology; Immunohistochemistry; Injections, Intraperitoneal; Lectins, C-Type/biosynthesis; Medulla Oblongata/chemistry/immunology/pathology; Mice; Mice, Inbred C57BL; Receptors, Cell Surface/biosynthesis; Research Support, Non-U.S. Gov't
AD Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Viale Regina Elena 299 00161, Rome, Italy.
SP englisch
PO Niederlande