NR ATCM
AU Fujita,R.; Urayama,T.; Ohmizu,A.; Yunoki,M.; Tsuji,S.; Ikuta,K.
TI Efficient detection of PrPsc (263K) in human plasma
QU Biologicals 2006 Sep; 34(3): 187-9
PT journal article
AB The potential contamination of human blood or plasma with prions, such as variant Creutzfeldt-Jakob disease (vCJD), is becoming a serious problem. In this study, we established a Western blot-based detection method for PrPsc (263K) spiked in plasma. Although plasma contains a large amount of protein, specific detection of a small amount of 263K in plasma could be specifically detected only after ultra-centrifugation followed by heat-denaturation of plasma proteins included in the resulting precipitate, before the digestion with proteinase K.
IN Die Autoren entwickelten eine auf Ultrazentrifugation, Hitzedenaturierung des Präzipitates und Western blot basierende Methode, mit der sie in Blutserum gemischtes PrPsc nachweisen konnten.
MH Blotting, Western/*methods; Creutzfeldt-Jakob Syndrome/*diagnosis; Humans; PrPsc Proteins/*blood; Protein Denaturation; Ultracentrifugation
AD Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan; Hirakata Laboratory, Research and Development Division, Benesis Corporation, Osaka, Japan.
SP englisch
PO England