NR ATCQ
AU Grosset,A.; Moskowitz,K.; Nelsen,C.; Pan,T.; Davidson,E.; Orser,C.S.
TI Rapid presymptomatic detection of PrPsc via conformationally responsive palindromic PrP peptides
QU Peptides 2005 Nov; 26(11): 2193-200
PT journal article
AB Structurally unique, synthetic prion peptides provide the basis of a simple assay to serve as both a detection and signal amplification system that distinguishes the normal prion protein, PrPc, from the misfolded prion protein, PrPsc, that is associated with the occurrence of transmissible spongiform encephalopathies (TSE). Proof-of-principle has been shown on brain samples from an experimental scrapie hamster model. The assay demonstrates very sensitive detection of PrPsc in animal brain tissue with potential application for early presymptomatic detection in animal screening. Furthermore, the sensitivity of the assay could enable blood tests for this TSE disease as well as other amyloid and/or misfolded protein diseases.
IN Leider ohne im Abstract irgend etwas zu erklären behaupten die Autoren, auf der Grundlage synthetischer Prionproteinpeptide einen einfachen, aber derart sensitiven TSE-Test entwickelt zu haben, dass dieser sich wahrscheinlich für frühe präklinische Diagnosen und sogar Bluttests eigne. Vor allem soll dieser Test auch zwischen PrPc und PrPsc unterscheiden.
MH Animals; *Biological Assay; *Brain Chemistry; Cricetinae; Female; Humans; Mesocricetus; Peptides/*analysis; PrPsc Proteins/*analysis; *Prion Diseases/diagnosis; *Protein Folding; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Sensitivity and Specificity
AD Department of Biochemistry and Molecular Biology, Georgetown University, Washington, DC 20006, USA
SP englisch
PO USA