NR ATDF
AU Kong,Q.; Huang,S.; Zou,W.Q.; Vanegas,D.; Wang,M.; Wu,D.; Yuan,J.; Zheng,M.; Bai,H.; Deng,H.; Chen,K.; Jenny,A.L.; O'Rourke,K.I.; Belay,E.D.; Schonberger,L.B.; Petersen,R.B.; Sy,M.S.; Chen,S.G.; Gambetti,P.
TI Chronic wasting disease of elk: transmissibility to humans examined by transgenic mouse models.
QU Journal of Neuroscience 2005 Aug 31; 25(35): 7944-9
IA http://www.jneurosci.org/cgi/content/full/25/35/7944
PT journal article
AB Chronic wasting disease (CWD), a prion disease affecting free-ranging and captive cervids (deer and elk), is widespread in the United States and parts of Canada. The large cervid population, the popularity of venison consumption, and the apparent spread of the CWD epidemic are likely resulting in increased human exposure to CWD in the United States. Whether CWD is transmissible to humans, as has been shown for bovine spongiform encephalopathy (the prion disease of cattle), is unknown. We generated transgenic mice expressing the elk or human prion protein (PrP) in a PrP-null background. After intracerebral inoculation with elk CWD prion, two lines of "humanized" transgenic mice that are susceptible to human prions failed to develop the hallmarks of prion diseases after >657 and >756 d, respectively, whereas the "cervidized" transgenic mice became infected after 118-142 d. These data indicate that there is a substantial species barrier for transmission of elk CWD to humans.
IN Die Autoren erzeugten Mauslinien, die keine Mausprionproteine, sondern entweder menschliche oder Wapiti-Prionproteine exprimieren. Während die Mäuse mit dem Hirschprionprotein 118-142 Tage nach intrazerebraler Inokulation erkrankten, waren die Mäuse mit menschlichen Prionproteinen selbst nach mehr als 657 bzw. sogar mehr als 756 Tagen noch gesund. Die Speziesbarriere zwischen Wapiti und Mensch scheint also einen relativ guten Schutz gegen CWD-Infektionen bei Menschen zu bieten.
MH Animals; Brain/pathology; Comparative Study; Deer; *Disease Models, Animal; Disease Transmission; Humans; Mice; Mice, Transgenic; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.; Survival Rate; Wasting Disease, Chronic/*genetics/mortality/pathology/*transmission
AD Qingzhong Kong, Shenghai Huang, Wenquan Zou, Difernando Vanegas, Meiling Wang, Di Wu, Jue Yuan, Mengjie Zheng, Hua Bai, Robert B. Petersen, Man-Sun Sy, Shu G. Chen, Pierluigi Gambetti, Department of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA; Huayun Deng, Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA; Ken Chen, Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA; Allen L. Jenny, National Veterinary Services Laboratories, United States Department of Agriculture, Ames, Iowa 50010, USA; Katherine O'Rourke, Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, Washington 99164, USA; Ermias D. Belay, Lawrence B. Schonberger, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
SP englisch
PO USA