NR ATER
AU Vetrugno,V.; Cardinale,A.; Filesi,I.; Mattei,S.; Sy,M.S.; Pocchiari,M.; Biocca,S.
TI KDEL-tagged anti-prion intrabodies impair PrP lysosomal degradation and inhibit scrapie infectivity
QU Biochemical and Biophysical Research Communications 2005 Dec 30; 338(4): 1791-7
PT journal article
AB Transmissible spongiform encephalopathy or prion diseases are fatal neurodegenerative disorders characterized by the conversion of the cellular prion protein (PrPc) into the infectious scrapie isoform (PrPsc). We have recently demonstrated that anti-prion intrabodies targeted to the lumen of the endoplasmic reticulum provide a simple and effective means to inhibit the transport of PrPc to the cell surface. Here, we report that they completely block the traffic of mature full-length PrPc molecules, impair prion lysosomal degradation, and interfere with the early phase of scrapie formation. Since anti-prion intrabodies efficiently block PrPsc accumulation in vitro, we investigated whether they could also antagonize scrapie infectivity in vivo. We found that mice intracerebrally injected with KDEL-8H4-NGF-differentiated PC12 cells infected with scrapie neither develop scrapie clinical signs nor brain damage. Furthermore, no protease-resistant PrPsc is detectable in brains of inoculated animals. These results indicate that anti-prion intrabody strategy may be effective against prion infection.
IN Die Autoren berichten über einen anscheinend erfolgreichen Versuch, durch die intrazerebrale Injektion bestimmter Zellen im Lumen des endoplasmatischen Retikulums wirksame sogenannte Antiprion-intrabodies in Stellung zu bringen und damit durch Blockierung des Transports reifen Prionproteins Mäuse immun gegen Scrapie-Infektionen zu machen.
MH Animals; Antibodies/*pharmacology; Intracellular Fluid/immunology; Lysosomes/*metabolism; Oligopeptides/*metabolism; PC12 Cells; PrPc Proteins/*metabolism; PrPsc Proteins/*pathogenicity; Prions/*immunology; Protein Sorting Signals; Rats; Research Support, Non-U.S. Gov't; Scrapie/*transmission
AD Department of Neuroscience and Laboratory of Clinical Biochemistry, University of Tor Vergata, Via Montpellier 1, 00133 Rome, Italy.
SP englisch
PO USA