NR ATFT
AU Deslys,J.P.; Comoy,E.E.
TI New insights in Prion decontamination
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Oral sessions ORAL-24
PT Konferenz-Vortrag
AB
The unique resistance of the vCJD/BSE agent to usual decontamination techniques poses a very difficult infection control challenge to health-care facilities, but also to the pharmaceutical industry and more widely to the food industry potentially exposed to the risk.
The drastic procedures recommended (1M sodium hydroxyde or 2% sodium hypochlorite for one hour, 134 C autoclaving for 18 min) are not applicable for fragile devices and numerous situations. Hence, new procedures need to be urgently developed to fill in this gap.
A rapid evolution of the recommendations implies a consensus on the validation methods which will be acceptable by health authorities. Different experimental models have been compared to evaluate the efficiency of classical prion decontamination methods versus new original procedures compatible with fragile devices. Reproducible results have been obtained in a 263K hamster model and a BSE mouse model of intracerebral infection by contaminated wires exposed to different treatments. We observed that limited concentrations of alcali or stabilized bleach, when properly formulated with detergents and used at specific temperatures, could exhibit similar efficiency on prions than the classical NaOH or NaOCl treatments. Short time exposures and compatibility with material render these treatments adapted for practical use. Similarly a dry system based on vaporized hydrogen peroxide under vacuum turned out to be more efficient than autoclaving whilst beeing compatible with electronic devices. These experimental models combined with in vitro protocols based on the detection of PrPres allow to investigate the effectiveness of new versus standard treatments and to explore the mechanisms of action involved.
Our conclusion is that new efficient procedures of prion decontamination can be evaluated in standardized experimental validation systems. As new protocols arise they should be considered for recommentation by official guidelines.
IN Die Autoren meinen, dass sich die Effektivität von Dekontaminationsmethoden sehr gut testen lässt, indem man Drähte mit 263K-Infektiosität kontaminiert, verschiedene Dekontaminationsmethoden auf die Drähte anwendet und diese dann in die Gehirne von Empfängerhamstern sticht. Mit dieser Methoden fanden sie neue Dekontaminationsmethoden, die schonender mit Oberflächen und sogar Elektronik umgehen. So reichen bei diesem speziellen Testsystem weniger konzentrierte Laugen, wenn man ihnen Detergentien zumischt und sie bei geeigneten Temperaturen verwendet. Elektronik soll sich schonend und sogar effektiver als durch Autoklavierung dekontaminieren lassen, wenn man sie mit einer dabei verdampfenden Wasserstoffperoxidlösung in ein Vakuum stellt.
AD J.P.Deslys, E.Comoy, CEA/DSV/DRM/GIDTIP, 18 route du Panorama, 92265 Fontenay-aux-Roses, France
SP englisch
PO Deutschland