NR ATHK
AU Evoniuk,J.M.; Johnson,M.L.; Caton,J.S.; Taylor,J.B.; Stoltenow,C.L.; O'Rourke,K.I.; Redmer,D.A.
TI Quantification of prion protein (PrPc) gene expression in the fetal and maternal sheep placenta
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Cell Biology of PrPc and PrPsc CELL-16
PT Konferenz-Poster
AB
Determining cellular prion protein (PrPc) expression in the placenta may increase our understanding of the transmission of scrapie, a transmissible spongiform encephalopathy of sheep. We examined PrPc mRNA expression in near-term (day 133) placentas of adolescent ewes that were fed adequate (n = 23) or nutrient-restricted (n = 27) diets (100% or 60% of recommended daily energy requirement, respectively) from day 90 to 133 of gestation. Gravid uteri (n=50) were collected at day 133 and total cellular (tc) RNA was isolated from samples of maternal (caruncle, CAR) and fetal placenta (cotyledon, COT). Cellular prion protein mRNA expression was quantified using real-time RT-PCR and ovine-specific oligonucleotide probes and primer sets. Prion protein (Prnp) genotypes for both fetal and maternal tissue were determined for codons 171 and 136 using SNP assays. COT had greater (P < 0.03) PrPc expression than CAR (1.31 ± .07 vs. 1.01 ± .10, respectively). The only genotype effect observed was in COT where expression of PrPc was greater (P < .002) when codon 136 was homozygous for alanine (1.41 ± 0.08; n = 40) relative to alanine valine genotypes (0.86 ± 0.07). Nutritionally restricted ewes tended to have greater (P < 0.11) PrPc expression (1.42 ± 0.11) than those receiving adequate nutrition (1.19 ± 0.08). We suggest that levels of expression of PrPc are associated with placental tissue type (i.e., COT vs. CAR), codon 136 Prnp genotype, and maternal nutritional level in late gestation and that further study is needed to determine their role in scrapie transmission via the placenta.
ACKNOWLEDGMENTS
Hatch Proj. ND1705 and USDA, ARS, ADRU.
IN Die Autoren fanden die mRNA für das Prionprotein in mütterlichen und fötalen Schichten der Schaf-Plazenta, wobei die Expression bei 136-Alanin-homozygoten größer als bei Alanin/Valin-heterozygoten Müttern, in der fötalen Plazenta größer als in der mütterlichen und bei unterernährten größer als bei gut ernährten Müttern war.
AD Jessica M. Evoniuk, Mary Lynn Johnson, Joel S. Caton, Charles L. Stoltenow, Dale A. Redmer, North Dakota State University, Fargo, ND, USA; J. Bret Taylor, USDA, ARS, USSES, Dubois, ID, USA; Katherine I. O'Rourke, USDA, ARS, ADRU, Pullman, WA, USA
SP englisch
PO Deutschland