NR ATIE
AU Paquet,S.; Chapuis,J.; Laude,H.; Vilette,D.
TI Prion spreading among living cells
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Cell Biology of PrPc and PrPsc CELL-36
PT Konferenz-Poster
AB
Although intercellular spreading of prion infection in infected tissues is a crucial step for prion dissemination through the body and pathogenesis, little is known on the mechanisms by which infectious prions are transferred from one cell to another. We have developed an experimental strategy enabling to visualise and study prion spreading in infected cell cultures. We used two different cell lines, the epithelial Rov cells and the neuroglial Mov cells, permissive to the same strain of sheep prion to show that i) the cell-to-cell spreading of infection may be an efficient phenomenon, as a whole culture of permissive target cells can be infected within 10 days by 10% of infected Mov cells, ii) the infection proceeds mainly step by step, through transmission to adjacent cells. However transmission to more distant cells is also suggested, iii) cell-to-cell infection is an active biological process, which could involve the release of infectious exosomes in addition to PrPsc/PrPc contacts between adjacent cells, iv) cell-to-cell spreading is much less efficient in permissive epithelial Rov cultures, suggesting that at least some factors controlling prion spreading are different from those conferring the ability to replicate the agent.
These experimental models should help to gain insights into the mechanisms involved in prion transmission among living cells and could be used to identify compounds interfering with cell-to-cell spreading of prions.
IN Die Autoren beobachteten in Zellkulturen, dass PrPsc-Infektionen sich hauptsächlich schrittweise (anscheinend über Exosomen) von Zelle zu Zelle ausbreiten, wobei die Ausbreitungsgeschwindigkeit bei verschiedenen Zelltypen sehr unterschiedlich ist.
AD Sophie Paquet, Jerôme Chapuis, Hubert Laude, Didier Vilette, INRA, Unité de Virologie Immunologie Moléculaires, Jouy en Josas, France
SP englisch
PO Deutschland