NR ATIN
AU Oelschlegel,A.M.; Geissen,M.; Groschup,M.H.
TI Infection studies to determine the susceptibility of cell lines to different TSE-strains
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Cell Biology of PrPc and PrPsc CELL-45
PT Konferenz-Poster
AB
Cell culture models are an essential part of the TSE research for the understanding of cellular and molecular processes leading to the formation and accumulation of the disease inducing agent, PrPres. Cell lines, susceptible for TSE and replicating PrPres, provide a basis for these studies. Unfortunately only a few of such cell lines exist until now and these are exclusively derived from the classical laboratory animals - mouse, hamster, rat and rabbit - infected with (foreseen by one exception) mouse adapted Scrapie strains.
No cell lines derived from a natural host species, e.g. an ovine cell line for Scrapie or even a bovine cell line for BSE, that were susceptible for natural occurring, not mouse adapted, TSE isolates, could be established so far.
In our study, we examined a variety of cell lines from different eucaryontic species, tissues and developmental stages for their susceptibility to several TSE strains. For this purpose we monitored the PrPc expression under different cell culture conditions (media, split ratios, etc.) and optimised the inoculation protocol as well as the detection methods.
AD Anja M. Oelschlegel, Markus Geissen, Martin H. Groschup, Friedrich-Loeffler-Institute / Federal Research Institute for Animal, Germany
SP englisch
PO Deutschland