NR ATIP
AU Bergström,A.L.; Berezin,V.; Lind,P.; Cordes,H.; Laursen,H.; Bock,E.; Heegaard,P.M.H.
TI Anti-prion effect of an NCAM-derived peptide
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Cell Biology of PrPc and PrPsc CELL-47
PT Konferenz-Poster
AB A synthetic fragment of the neural cell adhesion molecule (NCAM) named FGL binds to and activates the fibroblast growth factor receptor (FGFR). Through this, FGL has neuritogenic and neuroprotective functions. It was recently shown that FGL partially protects primary neurons from A-beta-25-35-induced killing and is able to dissolve cerebral amyloid plaques formed by synthetic A-beta-peptides injected intracerebrally in rats. Here, we tested the therapeutic potential of FGL against prion diseases. We found that FGL was able to significantly reduce the amount of PrPsc in chronically scrapie infected SMB cells (infected with two different strains of scrapie, s15 and F22, respectively). The same was observed with the polyene antibiotic Amphothericin B. However, FGL had no protective effect against PrP106-126-induced apoptosis in primary cultures of rat cerebellar granular cells. To test whether the mechanism, by which FGL decreases the amount of PrPsc in the SMB-cells is via FGFR signalling, we are currently treating the cells with a control peptide that resembles FGL, but is not able to bind to the FGFR. Furthermore, we are currently testing whether FGL can block the replication of PrPsc in vivo in RML-inoculated mice.
AD Ann-Louise Bergström, Peter Lind, Henriette Cordes, Peter M.H. Heegaard, The Danish Institute for Food and Veterinary Research, Denmark; Vladimir Berezin, Elisabeth Bock, The Protein Laboratory, University of Copenhagen, Denmark; Ann-Louise Bergström, Henning Laursen, The Neuropathology Laboratory, Rigshospitalet, Denmark
SP englisch
PO Deutschland