NR ATIV
AU Rapp,D.; Jocteur-Monrozier,L.; Potier,P.; Richaume,A.
TI TSE-soil fate: study of proteolytic activities in soil surrounding buried lamb carcasses
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-02
PT Konferenz-Poster
AB
This work is part of European Contract QLK4-CT-2002-02493
In several European countries, the Transmissible Spongiform Encephalopathy epidemic in cattle poses a major economic problem for the sustainability of breeding activities. The soil can be contaminated by the prion and become a potential reservoir of TSE infectivity as a result of the incorporation of animal tissues in fertilizers, accidental dispersion from storage plants of meat and bone meal, burial of carcasses of contaminated animals, etc. Whatever the contamination process, the prion protein is introduced into soil within a large quantity of other animal proteins. The latter may stimulate soil proteolytic activities, which could contribute to the degradation of the prion protein. However, the response of soils to animal protein input is difficult to predict mainly because the difference in the C/N ratio and the nature of animal proteins.
In order to have a better understanding of soil response to an input of animal proteins associated with the prion protein in natural situations, proteolytic activities of soil microorganisms were followed on a one year field experiment after lamb carcasses burying at various depth (10, 30, 90 cm). Soil samples were collected both above and below carcasses and proteolytic activities were measured using azocasein as substrate. It was shown that proteolytic activities stimulation occurred in all cases, even around carcasses buried at 90 cm. Furthermore, stimulation was always higher below than above carcasses. Finally, the dynamics of the proteolytic response was largely dependant on seasonal conditions.
In conclusion, these results are important parameters to take into account in further studies to evaluate PrP degradation in soil.
IN Bringt man beispielsweise durch das Vergraben eines toten Lammes große Mengen tierischen Proteins in einen Boden, dann stimuliert dies die proteolytische Aktivität des Bodens über und vor allem unter dem Kadaver, wobei das Ausmaß der Aktivität auch Saison-abhängig ist.
AD D.Rapp, L.Jocteur-Monrozier, P.Potier, A.Richaume, Ecologie microbienne, UCBL France
SP englisch
PO Deutschland