NR ATIX
AU Lawson,V.A.; Stewart,J.D.; Masters,C.L.
TI Development of an enzymatic detergent treatment protocol that significantly reduces protease resistant prion protein load and infectivity from prion contaminated surgical steel monofilaments.
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-04
PT Konferenz-Poster
AB
The unconventional nature of the infectious agent of prion diseases poses a challenge to conventional infection control methodologies. In this study PrPsc was used as a biochemical marker of prion infectivity to identify commercial enzymatic detergent preparations and conditions that could be used to degrade PrPsc present in homogenate and surface bound preparations of prion infected brain tissue. The ability of these treatment protocols to clear prion infectivity from contaminated surgical steel monofilaments was tested by in vivo bioassay in Tga20 indicator mice.
A high sensitivity quantitative western blot method was developed for the detection of PrPsc. Four commercially available preparations of enzymatic detergent (3M(TM) Rapid Multi-Enzyme Cleaner (RMEC) 70501 and 70505; Ruhof Endozime AW Plus; Steris Klenzyme) were tested for their ability to degrade PrPsc under conditions experienced in automated washing systems (0.8%, 43°, 5 minutes). All products tested failed to completely degrade PrPsc under these conditions. The effect of enzymatic detergent concentration, treatment time and temperature on PrPsc degradation was further optimised for RMEC 70501 and RMEC 70505. An extended treatment time (30 minutes) and higher temperatures (50-60°) decreased detectable levels of PrPsc from prion infected brain homogenates by more than 2 logs. Under these optimised conditions RMEC 70501 and RMEC 70505 completely removed detectable levels of PrPsc from prion contaminated surgical steel monofilaments. The incubation period of mice implanted with prion contaminated surgical steel monofilaments was extended when wires were treated under optimised conditions with RMEC 70501 and 70505 versus wires treated with 1N NaOH for 1 hour.
Optimisation of treatment protocols indicates that with extended treatment times and higher temperatures conventional enzymatic detergents can be used to reduce PrPsc load and prion infectivity from surgical steel instruments.
AD Victoria A. Lawson, James D. Stewart, Colin L. Masters, Department of Pathology, University of Melbourne, Australia und Mental Health Research Institute, University of Melbourne, Australia
SP englisch
PO Deutschland