NR ATJA
AU Lipscomb,I.P.; Sihota,A.; Keevil,C.W.
TI Development of a rapid visual and epi-microscopy techniques to assess the contamination of surgical instruments
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-07
PT Konferenz-Poster
AB The ability of traditional cleaning methods such as enzymatic, detergent and surfactant cleaning solutions in removing dried on surface contamination is difficult to accurately ascertain. Current methods for the detection of proteins or infectious organisms on surfaces largely depend on protocols that involve the sampling of the area of interest, with a swab or wipe, and the subsequent detection of any contamination on the collection material. This detection may involve traditional tissue culture techniques, or the application of other methods such as protein chemistry, enzyme immunoassay (EIA) or quantitative PCR (qPCR). However it is readily apparent that such approaches are inefficient, particularly on scratched surfaces, and are unlikely to detect very low levels of contamination. Indeed, the detection of low levels of prion protein contamination on the highly convoluted surfaces of surgical instruments, varying from forceps to endoscopes, poses particular problems. Here we describe the application of new developments in microscopy: utilising Episcopic Differential Interference Contrast / Epi-Fluorescence (EDIC/EF) techniques and the use of sensitive fluorescent dyes for the detection of sub-picogram amounts of protein and prion agent contamination on surgical metal instruments that have passed through traditional hospital trust sterile service regimes to validate their cleaning efficacy.
IN Die Autoren empfehlen eine episkopische differenzielle-Interferenz-Kontrastmikroskopie (Episcopic Differential Interference Contrast microscopy), mit welcher sich selbst winzigste (angeblich im Subpicogrammbereich) Proteinreste auf Oberflächen durch Interferenzen sehr schnell und hochsensitiv erkennen lassen sollen.
AD I.P.Lipscomb, A.Sihota, C.W.Keevil, Environmental Healthcare Unit, Southampton University, UK
SP englisch
PO Deutschland