NR ATJB
AU Walker,J.T.; Dickinson,J.; Dennis,M.J.; Sutton,J.M.; Fulford,M.; Hall,G.A.; Marsh,P.D.; Perrett,D.; Smith,A.; Raven,N.D.H.
TI Dental Procedures and the Transmission Risks of variant Creutzfeldt-Jakob Disease.
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-08
PT Konferenz-Poster
AB The theoretical risk of prion transmission via dental procedures is of current public health concern. Annually, an estimated 75 million dental procedures are conducted in the UK, of which 2 million are invasive root canal treatments. Studies have demonstrated that organic material is present on instruments post cleaning and sterilisation. Although it has not been possible to confirm epidemiologically that dental surgery is a risk factor in sporadic CJD transmission, possible instances of sCJD transmission have previously been identified. Studies have shown tissue distribution of vCJD infectivity (e.g. tongue) is much wider than with sporadic CJD, raising concerns over transmission of the prion protein via dental surgical instruments. Potential risks associated with vCJD and dentistry are being investigated. i) Bioassay results have indicated the effectiveness of the oral route for infectivity studies using a BSE301V infectious VM mouse model. Prion distribution and tissue infectivity is currently being investigated via this route, ii) A range of instruments including endodontic files, matrix bands, dental burs, forceps and scalers are being assessed for visual debris and for total protein using a fluorescent assay based on a reaction of proteins with o-phthaldehyde/N-acetyl cysteine to assess a range of cleaning regimens used in general dental practices (GDP) and central sterile services departments, and iii) A Taqman RT PCR assay has been developed to detect PrPc mRNA from saliva in water and is being used to assess human tissue contamination (PrPc mRNA) in dental unit water samples and aerosols from the dental surgery. This UK Department of Health funded programme aims to provide evidence for assessing public health risk and to develop practical methods to prevent iatrogenic transmission of vCJD via dental procedures.
AD James T. Walker, Joanne Dickinson, Mike Dennis, John M. Sutton, Graham Hall, Philip D. Marsh, Neil Raven, Health Protection Agency, UK; Martin Fulford, c/o Health Protection Agency, UK; David Perrett, Barts & the London School of Medicine & Dentistry, UK; Andrew Smith, University of Glasgow, Scotland, UK
SP englisch
PO Deutschland