NR ATJG
AU Riemer,C.; Mielke,M.; Baier,M.
TI Alkaline and phenolic cleaners/disinfectants for prion inactivation
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-13
PT Konferenz-Poster
AB A routinely usable alkaline cleaner for medical devices and two phenolic disinfectants were tested for their capacity to inactivate prions. Co-incubation of brain homogenates prepared from terminally ill scrapie-infected hamsters with the cleaner/disinfectants led to the denaturation of misfolded protein as proteinase K-resistant prion protein was no longer detectable after treatment. In addition, intracerebral inoculation of hamsters with neutralized cleaner/disinfectant-incubated samples reduced the level of infectivity of the material below the limit of detection. This report demonstrates the potency of a routinely usable alkaline cleaner and of phenolic disinfectants to reduce the infectivity of prions and to minimize the risk of iatrogenic transmissions of prion diseases by asymptomatic carriers. Our study encourages further investigations in this field.
IN Ein alkalisches Reinigungsmittel und zwei phenolische Desinfektionsmittel senkten die Infektiosität von Hirnhomogenaten Scrapie-kranker Hamster unter die Nachweisgrenze, wenn sie direkt mit den Homogenaten gemischt wurden. Auf dem Poster gezeigte Western blots zeigen aber, dass nach allen Behandlungen außer mit 0,5 M NaOH noch PrPc übrig geblieben war.
AD Constanze Riemer, Martin Mielke, Michael Baier, Robert Koch-Institute, Berlin, Germany
SP englisch
PO Deutschland