NR ATJH
AU Roth,K.; Yan,Z.X.; Heeg,P.; Stitz,L.; Gaegt,S.; Mauz,P.
TI Prions bound to stainless steel wires: An Instruments Processing Update
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-14
PT Konferenz-Poster
AB
Studies have shown that pathological prion protein is the agent that causes transmissible spongiform encephalopathies. The infectious prion proteins are found in and outside of the central nerve system, in lymphatic and non-lymphatic tissues. A number of studies have shown that prion proteins resist many conventional chemical and physical procedures used in hospitals for the processing of surgical instruments and medical devices. Currently there is concern among clinicians and health care professionals especially those involved in surgical procedures about the possibility of transmission of prion disease by surgical procedures.
There is an obvious need to develop new chemical and physical decontamination procedures to prevent such transmission. Especially for thermolabile instruments novel procedures have to be developed since those instruments can not be sterilized in an autoclave.
We report on an infectivity model using hamsters and prion protein contaminated steel wires to demonstrate transmission and the possibility of decontamination of infectious prion proteins. We show that the hamster adapted pathological prion proteins tightly bind to the steel wire and cause disease after the contaminated wires are implanted in the brain of hamsters. When the contaminated wires were treated with different reprocessing procedures before implantation infectivity was reduced which was manifested directly by prolonged survival time of the test animals.
Among those reprocessing procedures, the hydrogen peroxide gas plasma sterilizer (Sterrad), has been found effective against infectious prion proteins. Oxidative denature of prion protein can be used in combination with other disinfection agents as a non-autoclaving method for fighting iatrongenic TSE.
We have also succeeded in direct radioactive labelling of prion proteins, which paves a way for in vitro prion decontamination studies.
IN Mit einem Wasserstoffperoxid-Gasplasma-Sterilisierer von Sterrad konnten die Autoren effektiv die Infektiosität von Stahldrähten entfernen, die sie zur Kontrolle in die Gehirne von Empfängermäusen stachen. Als Ersatz für einen Teil der Übertragungsexperimente markieren sie Prionprotein auch radioaktiv, um so den Erfolg von Dekontaminationsmitteln zu untersuchen.
AD K.Roth, Z.X.Yan, P.Heeg, SMP GmbH Pruefen Validieren Forschen; Paul-Ehrlich-Strasse 40 72072 Tübingen, Germany; L.Stitz, Friedrich Loeffler Institut; Paul-Ehrlich-Strasse 28; 72076 Tuebingen, Germany; S.Gaegt, P.Mauz, Hals Nasen Ohren Klinik der Eberhard-Karls Universität; Elfriede Aulhorn-Strasse 5; 72076 Tuebingen, Germany
SP englisch
PO Deutschland