NR ATKV
AU Borthwick,E.B.; Herwig,R.; Janitz,M.; Hawkins,S.A.C.; Talbot,R.; Williams,J.L.
TI The search for differential gene expression in cattle spleen during early BSE infection
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-33
PT Konferenz-Poster
AB
Genes with differential expression between BSE infected and control animals have the potential to provide the means for early identification of the infection for disease monitoring. The route of infection, after oral transmission, of the infected PrPsc passes from the gastrointestinal system to the CNS via the lymporeticular system. Little is known of the effect on gene expression of the host organs during this early infection period. Although there is some evidence of decreased levels of erythroid differentiation-related factor (EDRF) in the spleen and erythroid cells in the blood of TSE infected mice. This opens up the possibility of using differential gene expression in peripheral tissues as an early diagnostic test for BSE infection.
In this project we are investigating the differential gene expression between normal and BSE infected cattle spleen. A bovine microarray was constructed to include a large population of immune biased genes. The 30k featured array includes 10k cDNAs from a 23k cDNA "non-redundant" bovine brain set isolated from a cDNA library constructed from brain stem, cerebellum, frontal cortex, and hypothalamus combined with 4.7k cDNAs from a normalised bovine macrophage array. Spleen samples were collected from Holstein cattle over a time range of 3 - 24 months post oral infection and from age matched controls. The RNA isolated from these spleens were used as targets on the microarray. After analysis a list of 56 significantly differentially expressed genes was produced. These results require verification by QPCR and classification into groups and families to enable a full understanding of the significance of these findings. These results will hopefully provide additional understanding of the TSE disease process and in the long term may lead to approaches to arrest or treat the disease at an early stage before irreparable damage has occurred.
AD Emma B. Borthwick, Roslin Institute, Roslin, Scotland; Ralf Herwig, Michal Janitz, Richard Talbot, John L. Williams, Max Plank Institute for Molecular Gentics, Berlin, Germany; Steve A.C. Hawkins, VLA Weybridge, Surrey, England
SP englisch
PO Deutschland