NR ATKZ
AU Göbel,K.A.; Schönenbrücher,H.; Abdulmawjood,A.; Bülte,M.
TI Comparative study on the detection of CNS-tissue by using three different detection methods: two ELISA-systems and a RT-Real Time-PCR
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-37
PT Konferenz-Poster
AB
EU prohibited several bovine and ovine tissues from food chain. To control the ban of bovine and ovine CNS-tissue and to offer a self-checking instrument for the food manufacturers, it is necessary to dispose a reliable detection method for CNS-contamination.
In this study three different methods for detection of CNS tissues in meat and meat products have been compared: the BrainosticTM ELISA-test (ScheBo(R), Giessen) and the RIDASCREEN(R) Risk Material 10/5 (R-Biopharm, Darmstadt) as well as a RT-Real Time-PCR system which was developed in our institute.
The two ELISA-systems are based on the detection of glial fibrillary acidic protein (GFAP), while the RT-Real Time-PCR uses a robust sequences region of the GFAP-messengerRNA as a CNS-specific marker.
As reference materials bovine minced meat was spiked with 0%; 0,1%; 0,2%; 0,5%; 1% and 5% of bovine brain and examined with the three systems at the day of reference material preparation and after 1, 3, 7 and 14 days. At the same time samples spiked with low concentrations of bovine spinal cord have been analysed
The first results of our studies will be presented including the signal strength during the storage. A special focus will be made on robustness and usability.
Key words: ELISA, RT-Real Time-PCR, detection of CNS-tissue
AD Katrin-Annette Göbel, Holger Schönenbrücher, Amir Abdulmawjood, Michael Bülte, Institute of Veterinary Food Science, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Germany
SP englisch
PO Deutschland