NR ATLC
AU Schönenbrücher,H.; Abdulmawjood,A.; Bülte,M.
TI Real Time RT-PCR for Species-Specific Detection of Central Nervous System Tissues as BSE Risk Material in Meat and Meat Products
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-40
PT Konferenz-Poster
AB
The emergence of a new variant of Creutzfeldt-Jakob disease (vCJD) during the bovine spongiform encephalopathy (BSE) epidemic has focused attention on the use of tissues from central nervous system (CNS) in food. For an efficient consumer's protection, European legislation prohibited specified bovine offals (SBOs), e. g. central nervous system (CNS) tissues, from food chain. Until now several chemical and immunological methods for the detection of tissues of the CNS in meat products have been published to be used in food safety control.
Our investigations were aimed at the design of a quantitative and species-specific real-time RT-PCR to identify BSE risk material in meat and meat products. This was based on an mRNA assay that used bovine, ovine, and caprine glial fibrillary acidic protein (GFAP) encoding gene sequences as a marker. The developed real-time RT-PCR assay allowed the reliable detection of CNS tissues of the mentioned ruminant animal species in meat and meat products. Bovine brain at a concentration of 0.1% yielded a positive PCR reaction. The detection was neither affected by different heat treatments of the product nor tissue samples of various animal origins. Detailed data on the estimation of tissue-specificity will be presented.
The quantitative real-time RT-PCR detection of GFAP mRNA appears to be useful as a routine diagnostic test for the detection of illegal use of CNS tissues in meat and meat products. The stability of the specific region of GFAP-mRNA allows the detection of CNS tissues also after meat processing steps. The use of organ and species specific subunits of mRNA might be a promising approach also for the detection of other banned tissues.
This work is a part of a project including three German veterinary institutes at the Justus-Liebig-University Giessen and is founded by the Federal Ministry of Consumers Protection, Food and Agriculture (AZ.: 01HS022/1).
Key words: Real Time-PCR, species-specific detection of BSE-risk material
AD ger Schönenbrücher, Amir Abdulmawjood, Michael Bülte, Institute of Veterinary Food Science, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Germany
SP englisch
PO Deutschland