NR ATLF
AU Monleon,E.; Acin,C.; Bolea,R.; Vivanco,V.; Hortells,P.; Vargas,A.; Monzon,M.; Badiola,J.J.
TI Animal tissue bank of the University of Zaragoza
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-43
PT Konferenz-Poster
AB
The establishment of animal repositories is one of the NeuroPrion network objectives, the tissue bank at the National Reference Centre for TSEs of the University of Zaragoza being involved in this task.
The bank is supplied by samples from the National TSE surveillance programme (nervous tissue, mainly fixed, from BSE and scrapie positive animals) and from a monitored sheep and goats naturally infected scrapie flock. Concerning this flock:
- PrP genotype presented by each animal has been determined.
- A clinical examination and lymphoreticular tissue biopsies are periodically performed in order to determine the infection status of the animals and classify them depending on the clinical stage of the disease (preclinical, clinical or terminal). Furthermore, samples (fluids and faeces) from all positive animals are gathered and stored at regular intervals.
- Tissues belonging to the central nervous, lymphoreticular (LRS) and other systems as well as different fluids and faeces are collected from all the animals at the moment of necropsy. Fluids are kept frozen, half of each tissue sample is stored frozen and the other half in formalin / paraffin embedded. Histopathological examination and PrPsc immunodetection is performed in each collected tissue.
Additionally, LRS biopsies from animals belonging to other different scrapie positive flocks detected in the frame of the National surveillance programme are currently analysed and all those animals presenting a positive result are included in the monitored flock.
In conclusion, the Spanish Reference Centre for TSEs tissue bank keeps samples and information from BSE and scrapie cases that offers to the European research community.
ACKNOWLEDGMENTS
The authors thank the NeuroPrion network and Institute of Health Carlos III (CIEN network) for financial support and National Reference Centre for TSEs staff for technical assistance.
AD Eva Monleon, Cristina Acin, Rosa Bolea, Virginia Vivanco, Paloma Hortells, Antonia Vargas, Marta Monzón, Juan José Badiola, National Reference Centre for TSEs
SP englisch
PO Deutschland