NR ATLG
AU Lulini,B.; Corona,C.; Mazza,M.; Perazzini,A.Z.; Nonno,R.; Di Bari,M.A.; Ligios,C.; Simmons,M.M.; Acutis,P.L.; Caramelli,M.; Casalone,C.
TI Immunohistochemical and molecular analysis of pathological prion protein in Italian scrapie - affected goats
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-44
PT Konferenz-Poster
AB
Studies on neuropathological and immunobiochemical features of scrapie are limited in goats, because the low prevalence of the disease has afforded few infected animals. The interest in transmissible spongiform encephalopathies in goats has recently increased after the report of a natural case of BSE in a French goat.
In Italy, scrapie in goats was first described in 1997 and since then 26 outbreaks involving goats have been reported. We report here a detailed study of the type and immunohistochemical distribution of PrPsc in CNS, and on the molecular analysis of PrPsc in Italian TSE affected goats. We collected 63 animals from 16 different outbreaks. Immunohistochemistry was performed on the obex and, in 40 animals for which the whole brain was available, on six additional neuroanatomical levels. For 23 animals, it was also possible to carry out a Western blot analysis on frozen material from the obex. Ten morphologically different PrPsc types were identified immunohistochemically and scored for intensity and distribution within defined neuroanatomical sites. In seven cases several plaque-like deposits were found, mainly in the mesencephalon and the thalamus. Western blot analysis of these goats revealed consistent immunobiochemical features i.e. the molecular weight of PrPsc unglycosylated band, the glycoform ratio and the differential staining using MAb 6H4/P4. No cases with BSE-like features were identified.
IN
Die Autoren berichten, dass in Italien der erste Scrapie-Fall bei einer Ziege erst 1997 gefunden worden sei. Das spricht eher nicht für die Effektivität der damaligen TSE-Überwachungs, denn seitdem wurden dort 26 Scrapie-Ausbrüche gezählt, bei denen auch Ziegen betroffen waren.
Die Autoren untersuchten immunhistochemisch von 63 Ziegen aus 16 Ausbrüchen den Obex und von 40 Ziegen auch andere Teile des Gehirns. Bei 23 Ziegen war es außerdem möglich, Obex-Gewebe mittels Western blot zu untersuchen. Immunhistochemisch konnten die Autoren 10 Typen hinsichtlich der Verteilung und Menge des PrPsc unterscheiden. Hinweise auf BSE in Ziegen fanden sie nicht.
AD Barbara Lulini, Cristiano Corona, Maria Mazza, Alice Z Perazzini, Pier Luigi Acutis, Maria Caramelli, Cristina Casalone, Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Italy; Romolo Nonno, Michele di Bari, Istituto Superiore di Sanità, Italy; Ciriaco Ligios, Istituto Zooprofilattico Sperimentale della Sardegna, Italy; Marion M Simmons, Veterinary Laboratories Agency, UK
SP englisch
PO Deutschland