NR ATLL
AU Zhang,W.L.; Becker,K.; Karch,H.; Peters,G.; Groschup,M.H.; Kuczius,T.
TI Sensitive Detection of Low Levels of Prion Proteins by the Immuno-PCR Technique
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-49
PT Konferenz-Poster
AB Prion diseases are fatal disorders of the central nervous system that occur in humans as Creutzfeldt-Jakob disease and in animals as bovine spongiform encephalopathy (BSE) of cattle and as scrapie of sheep and goats. A common feature of the disease is the neuronal accumulation of a pathological isoform (PrPsc) of the host encoded cellular prion protein (PrPc). Prion proteins are detected by antibody-based tests to confirm the disease. However, sensitivity and specifity are the limiting factors of these techniques referring to sufficiently accumulated PrPsc in brain. There is great demand on developing new techniques detecting as well PrPc as PrPsc at very low amounts in tissues, body fluids and blood. We established a very sensitive and specific quantitative real-time immuno-polymerase chain reaction (Immuno-PCR) assay combining the specificity of an immunological detection with an exponential amplification by PCR and assessing the maximal sensitivity. The specific signals are intensified by amplification of bound target DNA molecules using quantitative PCR in a LightCycler. This assay attained a 1000-fold increased sensitivity compared with results of the ELISA technique. The use of the high-sensitive quantification Immuno-PCR method with a significant increase of the efficiency in the prion protein analytic may be achievable as an early stage diagnosis of prion diseases and of product contamination.
AD W.L.Zhang, H.Karch, T.Kuczius, Insitute for Hygiene, University Hospital Münster; K.Becker, G.Peters, Institute for Medical Microbiology, University Hospital Münster; M.H.Groschup, Friedrich-Loeffler-Institute, Institute for Novel and Emerging Infectious Diseases
SP englisch
PO Deutschland