NR ATLV
AU Cooper,J.K.; Ladhani,K.; Fitt,N.A.; Minor,P.D.
TI Reference materials for human TSE research and diagnosis
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-59
PT Konferenz-Poster
AB
The National Institute for Biological Standards and Control provides standards and reference materials for laboratories testing for blood borne pathogens. Under the auspices of the World Health Organization it was determined that there is a need for reference materials for human TSE research and diagnosis. Within the last two years there have been two reported transmissions of vCJD through blood donation, leading to an increased urgency for a test detecting vCJD infectivity in blood.
Reference reagents have been prepared from brain: two vCJD cases; sporadic CJD type 1 (MM 129 genotype); sporadic CJD type 2 (MM 129 genotype); sporadic CJD type 1 (MV 129 genotype) and control brain (MM genotype). . Homogenates have also been prepared from spleens of two vCJD cases and a control. All have been characterized for their PrPsc content.
Several TSE assays are approaching the sensitivity needed to detect infectivity in blood. There is however very limiting supplies of blood from vCJD cases available for testing. In the absence of available blood we have proposed two independent ways of determining the sensitivity of TSE infectivity assays: one is to generate a panel of vCJD and control tissue homogenates diluted in human plasma and the other is to source material from animal models of TSE disease.
Blood, brain and spleen tissues have been collected from animals suspected of TSE disease (two scrapie cases and a BSE suspect).
AD Jillian K. Cooper, Kaetan Ladhani, Natasha A. Fitt, Philip D. Minor, CJD Resource Centre, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, UK-Hertfordshire EN6 3QG. UK
SP englisch
PO Deutschland