NR ATLW
AU Ladhani,K.; Minor,P.D.; Cooper,J.K.
TI Recombinant Human 14-3-3 gamma for use in Sporadic CJD Diagnosis
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-60
PT Konferenz-Poster
AB
Creutzfeldt-Jakob disease (CJD) is a neurodegenerative prion disease that causes loss of movement, cognition, and mental status. The clinical heterogeneity of the disease can make diagnosis difficult. Hence, accurate biological markers both for confirming and excluding CJD would be of great value. The currently recommended investigation of a patient with possible CJD comprises clinical evaluation and is supported by a positive 14-3-3 Western blot of cerebrospinal fluid (CSF).
CSF 14-3-3 is present in >80% of sporadic CJD cases but only 50% of vCJD cases (Green 2002). Of the seven isoforms of 14-3-3 five are abundantly expressed in brain (ß, epsilon, gamma, eta, zeta).
Here we describe the development of a purified recombinant 14-3-3 gamma protein sample that can be run alongside patient CSF samples to act as a positive control for the diagnosis of neurodegenerative diseases.
Green, A. J. (2002). "Use of 14-3-3 in the diagnosis of Creutzfeldt-Jakob disease." Biochem Soc Trans 30(4): 382-6.
AD Kaetan Ladhani, Philip D. Minor, Jillian K. Cooper, CJD Resource Centre, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, UK-Hertfordshire EN6 3QG. UK
SP englisch
PO Deutschland