NR ATMF
AU Acutis,P.L.; Bossers,A.; Priem,J.; Peletto,S.; Colussi,S.; Martucci,F.; Casalone,C.; Forloni,G.; Ru,G.; Caramelli,M.
TI Prion protein gene polymorphisms in goats from Italian scrapie outbreaks
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-05
PT Konferenz-Poster
AB Susceptibility to scrapie in sheep is influenced by polymorphisms of the prion protein (PrP) gene, while no strong association between genetics and scrapie has been determined in goats, due to the limited number of studies. Here a case-control study is described, carried out on 177 goats, from six Italian scrapie outbreaks, in order to look for association between PrP alleles and the occurrence of scrapie. PrP polymorphisms were detected by direct DNA sequencing on both strands of the PCR products. To be able to link detected polymorphisms in PrP into an allele sequence (haplotype), PCR amplified products of selected samples were cloned in a TA-cloning vector. "Chi square" test was performed to look at the association between each allele and the scrapie status. This was carried out by comparing the frequencies of genotypes with and without an allele between cases and controls: heterozygotes and homozygotes for the same allele were combined in one single group. When the available numbers permitted a multivariate analysis to be carried out, logistic regression was used in order to account for confounding from age and flock. Eleven PrP polymorphisms were identified, two of which are novel (L133Q, M137I). By cloning, 12 alleles have been determined. Statistical analysis showed no association between the alleles and the scrapie status except for two alleles. Allele with polymorphism S240P resulted to be associated with an increased risk of disease: this positive effect was still evident in the multivariate analysis after adjusting for age but loosed its statistical significance when the logistic model included the flock as a covariate. The allele with mutation Q222K instead showed a significant association with healthy animals, suggesting a possible protective role against scrapie in goats.
AD Pierluigi Acutis, Simone Peletto, Silvia Colussi, Francesca Martucci, Cristina Casalone, Giuseppe Ru, Maria Caramelli, CEA-Italian Reference Centre for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta; Gianluigi Forloni, Istituto di Ricerche Farmacologiche Mario Negri, Italy; Alex Bossers, Jan Priem, Central Institute for Animal Disease Control (CIDC-Lelystad), The Netherlands
SP englisch
PO Deutschland