NR ATMJ
AU Bossers,A.; Harders,F.L.; Smits,M.A.; van Keulen,L.J.M.; van Zijderveld,F.G.
TI Identification of early natural scrapie-specific gene expression changes in tonsil and Peyer's patches of sheep using a generated sheep cDNA microarray
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-09
PT Konferenz-Poster
AB
All current routine diagnostics for TSEs are thus far based solely on the detection of pathogically folded prion protein (PrP). In addition, the process of TSE agent-uptake which takes place in the gut via the ileal Peyer's patches is still poorly understood, as is the host response to the TSE agent. This research focuses on the identification of new biomarkers (other than PrP) that might be useful to extend current TSE diagnostics. Furthermore, we studied the host response / gene expression profiles to a natural scrapie infection in sheep.
We have generated a cDNA microarray of about 32.500 spots per slide containing 7.369 unique features from a large EST library of Tonsil and Peyer's patches of sheep (non-infected and infected of different PrP genotypes). The unique features were determined by sequencing, clustering and assembling over 13.000 ESTs into 1.850 contigs and 2.594 singletons. Only about 28% for these sequences could be 'annotated' by BLAST to publicly available databases thus far.
To probe the sheep arrays we collected and mRNA preserved many different tissues that include tonsil, Peyer's patches of ileum and jejunum, brain regions, blood, muscle regions and RLNs. These materials were collected at different time points (0, 3, 8 and 32 weeks) of sheep having different PrP genotypes after natural exposure to scrapie or from scrapie-free sheep. Tissues used for array hybridisation were also checked by IHC for scrapie to be able to link the found gene expression differences to the kinetics of PrPsc deposition. Preliminary results of the gene expression profiling will be presented.
AD A.Bossers, F.L.Harders, L.J.M.van Keulen, F.G.van Zijderveld, Central Institute for Animal Disease Control Lelystad (CIDC-Lelystad), The Netherlands.; M.A.Smits, Animal Sciences Group, Wageningen UR, The Netherlands
SP englisch
PO Deutschland