NR ATMM
AU Schiller,I.; Duss,J.; Kuhn,F.; Schmid,J.; Glatzel,M.; Ehrensperger,F.; Hilbe,M.; Zlinszky,K.; Stierstorfer,B.; Oesch,B.; Raeber,A.J.
TI A special form of PrPsc in muscles of a BSE-infected cow
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-12
PT Konferenz-Poster
AB
PrPsc, the abnormal form of the prion protein, has been detected in muscles of patients with sporadic Creutzfeldt-Jakob disease, in natural and experimental scrapie in sheep and in rodent adapted scrapie but not in cattle with bovine spongiform encephalopathy (BSE).
In this study we have analyzed 60 muscle samples originating from 7 Swiss and 6 Bavarian cows with confirmed BSE by 3 different methods: (1) phosphotungstic acid precipitation followed by proteinase K digestion, (2) phosphotungstic acid precipitation followed by immuno-precipitation with the PrPsc conformation specific antibody 15B3, and (3) ultracentrifugation followed by immunoprecipitation with the antibody 15B3. Analysis of PrP was performed by Western blotting with the antibody 6H4. Here we report for the first time the detection of a special form of PrPsc in muscles of a clinical BSE case. This form of PrPsc is protease-resistant yet the protease-resistant fragment detected on Western blot differs from that typically found in BSE positive brains. In 1 of 7 Swiss BSE cases, we detected PrPsc in semitendinous muscle, triceps muscle, sternocephalicus muscle and in the tongue but not in two other muscles analysed. Our findings show that low levels of a special form of PrPsc are present in a small percentage of muscles from BSE affected cattle. Whether this type of PrPsc in muscle of cattle with BSE is associated with infectivity remains to be established.
AD I.Schiller, J.Duss, F.Kuhn, J.Schmid, B.Oesch, A.J.Raeber, Prionics AG, Switzerland; M.Glatzel, F.Ehrensperger, M.Hilbe, K.Zlinszky, Universitiy of Zürich, Switzerland; B.Stierstorfer, Ludwig-Maximilians-Universität Muenchen, Germany
SP englisch
PO Deutschland