NR ATMS
AU Martin,S.; Jeffrey,M.J.; Gonzalez,L.; Foster,J.; Langeveld,J.P.M.; van Zijderveld,F.G.; Grassi,J.; Hunter,N.
TI Immunohistochemical Features of PrPd Accumulation in Natural and Experimental Goat Transmissible Spongiform Encephalopathies
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-18
PT Konferenz-Poster
AB
Scrapie is a transmissible spongiform encephalopathy (TSE) or prion disease, which naturally affects sheep and goats. Immunohistochemical epitope mapping of abnormal PrP accumulations (PrPd) in brain can help the characterisation of sheep TSE sources or strains, and particularly, the identification of potential bovine spongiform encephalopathy (BSE) infections of sheep. In the present study we have examined natural and experimental TSE infections of goats to determine whether the epitope mapping approach could also be applied to aid recognition of goat BSE infection.
Goats experimentally infected with the SSBP/1 or CH1641 sheep scrapie strains or with cattle BSE and four field cases of natural TSE in goats were examined by immunohistochemistry using 6 different antibodies. Goat CH1641 and SSBP/1 infections were similar to their parental sheep strains insofar as they predominantly resulted in intracellular PrPd accumulations. Some differences in targeting, particularly to Purkinje cells, were evident in inter-species comparisons of CH1641 and SSBP/1 infections. PrPd labelling of goat BSE experimental cases showed extensive intracellular and extracellular accumulations also similar to those of experimental sheep BSE. Intra-neuronal PrPd in both goat and sheep BSE was only labelled by antibodies recognising epitopes located C terminally of residue His99, whereas in natural sheep TSE sources and in sheep and goat SSBP/1, PrPd was also detected by antibodies to epitopes located N-terminally from residue Ser100.
Testing of four natural goat TSE samples showed one case with epitope mapping characteristics and overall pattern of PrPd accumulation identical to that of experimental goat BSE. According to these parameters, the other three natural goat TSE cases were scrapie-like, but showed some degree of immunohistochemical phenotype variability suggesting that multiple strains may exist within the relatively small UK goat population.
IN Die Autoren verglichen immunhistochemisch 4 im Vereinigten Königreich gefundene Fälle von TSE bei Ziegen mit Ziegen, die experimentell entweder mit BSE oder mit zwei verschiedenen Scrapie-Stämmen infiziert wurden. Drei der vier Ziegen ähnelten den experimentellen Scrapie-Fällen, aber die Hirnschnitte einer Ziege sah genauso aus wie bei experimentellem Ziegen-BSE.
AD S.Martin, M.Jeffrey, L.Gonzalez, Veterinary Laboratories Agency (VLA) - Lasswade, UK; J.Foster, N.Hunter, Institute for Animal Health, NPU - Edinburgh, UK; J.P.M.Langeveld, F.G.van Zijderveld, Central Institute for Animal Disease Control - Lelystad, Netherlands; J.Grassi, CEA Service Farmacologie et Immunologie - Saclay, France
SP englisch
PO Deutschland