NR ATNQ
AU Montag,J.; Hunsmann,G.; Schulz-Schaeffer,W.J.; Motzkus,D.
TI Transmission of BSE to cynomolgus macaques
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Human prions, risk of blood products, and therapy HUMAN-14
PT Konferenz-Poster
AB
The risk of transmitting transmissible spongiform encephalopathies (TSEs) from animal to humans is still an important question in TSE research. There are scientific indications that bovine spongiform encephalopathy (BSE) is transmitted to humans causing variant Creutzfeldt Jakob disease (vCJD). Previous studies have shown that infectious material from cattle can cause TSEs in cynomolgus macaques (M. fascicularis) resembling the lesion profile of vCJD. Recently, oral infection using macaque-adapted brain homogenate was successful in the same animal model.
In cooperation with five European partners a quantitative study for the transmission of the BSE agent to M. fascicularis was initiated to assess the risk of vCJD infection in humans through contaminated of BSE-infected material. Infection was performed orally and, as a control, intracerebrally with non adapted BSE material. As the first result we report the successful intracerebral transmission of BSE to M. fascicularis. 6/6 have died of TSE. The analysis of one animal is exemplified here. By immunohistology, amyloid plaques with PrP depositions were detected in different brain sections. The Western Blot analysis revealed PrP-specific bands between 26 and 39 kDa. After PK-digestion of the infected macaque brain homogenate this pattern shifted to three bands between 17 - 36 kDa. The bandshift was also detected after PNGase digestion. These data confirm the clinical diagnosis of vCJD.
IN Die Autoren inokulierten auch Javaneraffen genannte Langschwanzmakaken (Macaca fascicularis, Crab-eating Macaque, Cynomolgus Monkey oder Long-tailed Macaque) intracerebral und oral mit BSE. Inzwischen sind alle 6 intrazerebral inokulierten Affen erkrankt und zeigten im Western blot ähnliche Muster wie nvCJK-Patienten.
AD J.Montag, G.Hunsmann, D.Motzkus, German Primate Centre, Department of immunology and virology, Göttingen, Germany; W.Schulz-Schaeffer, Georg-August-Universität Göttingen, Dept. of Neurology, Robert-Koch-Str. 40, 37075 Göttingen, Germany
SP englisch
PO Deutschland