NR ATNX
AU Siso,S.; Gonzalez,L.; Houston,E.F.; Foster,J.; Martin,S.; Hunter,N.; Jeffrey,M.J.
TI The pathological phenotype of sheep BSE is not altered following blood transfusion
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Human prions, risk of blood products, and therapy HUMAN-21
PT Konferenz-Poster
AB
Infectivity has been demonstrated in both red and white cell fractions of the blood, and intra-venous transmission of BSE, vCJD and scrapie have been described. We report here the pathological PrPd phenotypes of sheep TSEs arising from blood transfusions from BSE donors (n=3) and natural scrapie (n=5) cases to 5 ARQ/ARQ and 6 VRQ/VRQ sheep recipients, respectively. All transfusions were carried out with whole blood except for 1 BSE and 3 scrapie cases, in which the leukocyte fraction was used. Six sheep intravenously infected with BSE brain homogenate were used as positive controls.
All recipients were allowed to develop clinical signs, and incubation periods were shorter for BSE than for scrapie recipients. The total amounts of PrPd detected by immunohistochemistry in brain were similar in BSE and scrapie recipients, whereas scrapie recipients showed greater levels of PrPd in lymphoreticular (LRS) tissues. In general, recipients showed higher PrPd deposition than their donor counterparts. Transmission was achieved from donors which were either clinically or subclinically affected at the time of donation; some of these last did not show any PrPd in brain or LRS tissues.
The PrPd profiles in the brain of BSE donors (orally challenged) and their recipients were very similar and characteristic of experimental sheep BSE, as were those of the BSE positive controls. This finding agrees with previous observations on intracerebral BSE transmission to sheep of different PrP genotypes. In contrast, some variability was detected in the PrPd profile within and between scrapie donors (naturally infected) and recipients.
We conclude that while the phenotype of ovine BSE is maintained after blood transfusion, the variability observed for scrapie might be attributable to the presence of more than one strain within the flock of naturally infected donors.
IN Die Verteilungs- und Schädigungsmuster sind bei intrazerebral und intravenös mit BSE infizierten Schafen praktisch gleich und von Infektionen zumindest mit den bisher daraufhin untersuchten Scrapie-Stämmen unterscheidbar.
AD S.Sisó, L.González, S.Martin, M.Jeffrey, Veterinary Laboratories Agency (VLA) - Lasswade; F.Houston, Institute for Animal Health (IAH)- Compton; J.Foster, N.Hunter, IAH-Neuropathogenesis Unit- Edinburgh
SP englisch
PO Deutschland