NR ATOM
AU Lefebvre-Roque,M.; Demercieres,J.; Sales,N.; Schätzl,H.M.; Kremmer,E.; Lasmezas,C.I.
TI Neuropathological evaluation of intracerebroventicular administration of anti-PrP antibodies on the progression of bovine spongiform encephalopathy in mice.
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Human prions, risk of blood products, and therapy HUMAN-36
PT Konferenz-Poster
AB
Absence of specific immune response is a hallmark of prion diseases. However, in vitro and in vivo experiments have provided strong evidence that an anti-PrP humoral response could have beneficial effects and could represent a new therapeutic approach. Passive immunizations before neuroinvasion have been shown to delay or prevent disease. Nonetheless, potential therapeutic effects of antibodies on central nervous system (CNS) pathogenesis have never been tested.
The aim of the present study was to evaluate the effect of anti-PrP antibody administration once the infectious agent has entered the CNS to assess the possibility to impede or halt the neurodegenerative process. To this purpose, we have inoculated intraperitoneally Tga20 mice with a mouse adapted BSE strain (6PB1). At the preclinical stage (85 days post-inoculation), we have administered anti-PrP antibody 4H11 or 4H11 F(ab')2 fragments by intracerebroventricular route continuously during 2 weeks using ALZET(R) osmotic pump system implantation.
The treatment did not augment significantly survival time. The results will be interpreted in the light of the effect of the antibody treatment on PrPsc accumulation and neurodegenerative parameters like neuronal death, microglial and astrocytic reaction in mice sacrificed at the end of the treatment period.
AD Maxime Lefebvre-Roque, Jeremy Demercières, Nicole Salès, Corinne I. Lasmezas, CEA, Fontenay-aux-Roses, France; Hermann Schätzl, Institute of Virology, Technical University of Munich, Munich, Germany; Elisabeth Kremmer, Institute of Immunology, Technical University of Munich, Munich, Germany
SP englisch
PO Deutschland