NR ATOQ
AU Vidal,E.; Marquez,M.; Tortosa,R.; Costa,C.; Serafin,A.; Pumarola,M.
TI Insights in the pathogenesis of Bovine Spongiform Encephalopathy in its early stages
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-01
PT Konferenz-Poster
AB
An immunohistochemical and histochemical study has been carried out on the brains of 9 cases of BSE diagnosed in cattle in the TSE surveillance plan in Catalonia, Spain. The animals did not have any clinical symptoms reported and were thus at early stages of the disease.
A characterization of PrPbse deposits throughout the encephalon has been performed. An immuno-labelling pattern tropism has been observed towards certain brain areas. This tropism is principally directed to the brain stem region; however, an association of the stellate pattern has been found with areas where PrPbse is less abundantly deposited, such as the cerebral cortex.
Furthermore we have investigated distinct pathogenetic mechanisms taking place in these early stages of BSE. We have observed a glial response associated to the presence of PrPbse (using antibodies against astrocytic glial fibrillary acidic protein and lectin from Griffonia simplicifolia to identify microglia), the presence of oxidative stress phenomena (antibodies against metallothioneins I and II and against nitrated aminoacidic residues: nitrotyrosine). We have also studied apoptotic cellular death (cleaved caspase 3) and the state of the synaptic proteins synaptophysin and small synaptosome-associated 25KD protein. Finally, the status of the extra-cellular matrix has been evaluated using Wisteria floribunda agglutinin, a marker for perineuronal nets.
This study was partially financed by the project with reference ET2002-05168-C04-01 of the Spanish Ministry of Science and Technology (MCyT).
AD Enric Vidal, Martí Pumarola, Priocat Laboratory, CReSA, Universitat Autònoma de Barcelona, Spain; Mercedes Márquez, Martí Pumarola, BTAC, Department of Animal Medicine and Surgery , Veterinary Faculty, Universitat Autònoma de Barcelona, Spain; Raül Tortosa, Carme Costa, Anna Serafin, Martí Pumarola, Department of Animal Medicine and Surgery , Veterinary Faculty, Universitat Autònoma de Barcelona, Spain
SP englisch
PO Deutschland