NR ATOS
AU Costa,C.; Bassols,A.; Tortosa,R.; Vidal,E.; Diaz San Segundo,F.; Torres,J.M.; Brun,A.; Pumarola,M.
TI Assessment of the central nervous system extracellular matrix in a transgenic murine model of BSE
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-03
PT Konferenz-Poster
AB
Brain extracellular matrix (ECM) is known to be disturbed in certain neurodegenerative disorders. In human TSE studies and murine scrapie models, ECM alterations have been described in association with PrPsc deposition and neurodegenerative lesions. Because of its expression on the outer surface of the cellular membrane, PrP interaction with ECM components has been pointed out in several in vitro studies.
Transgenic mice (boTg110 line, overexpressing bovine cellular PrP) have been intraperitoneally inoculated with BSE1 (a standardised pool of BSE infected cattle brains: TSE/08/59 from VLA, U.K.) and sacrificed at different times post inoculation. Mice have been perfused with paraformadehide, and, after removal, the brains were postfixed and paraffin embedded for their histopathological examination.
Several ECM components have been evaluated in this model by means of affinity histochemistry (Wisteria floribunda agglutinin and Hyaluronic Acid Binding Protein) and immunohistochemistry techniques (anti-aggrecan and tenascin-R polyclonal antibodies). The brain distribution of the named ECM markers has been thoroughly evaluated in the studied model as well as in its evolution during infection.
The present results are the first description of the distribution of these ECM components in the mouse brain during BSE infection.
This study was financed by the project with reference ET2002-05168-C04-01 of the Spanish Ministry of Science and Technology (MCyT).
AD Carme Costa, Raül Tortosa, Martí Pumarola, Animal medicine and Surgery Department, Veterinary Faculty, Universitat Autònoma de Barcelona, Spain; Martí Pumarola, Animal Tissue Bank of Catalonia (BTAC) , Spain; Enric Vidal, Martí Pumarola, Priocat Laboratory, CReSA, Universitat Autònoma de Barcelona, Spain; Fayna Diaz San Segundo, Juan Maria Torres, Alejandro Brun, Animal Health Research Center (CISA-INIA), Spain; Anna Bassols, Biochemistry an Molecular Biology Department, Veterinary Faculty, Universitat Autònoma de Barcelona, Spain
SP englisch
PO Deutschland